phase contrast inverted microscope carl zeiss axio observer z1  (Carl Zeiss)

Journal: Microbiology Spectrum

Article Title: Blocking the shikimate pathway amplifies the impact of carvacrol on biofilm formation in Candida albicans

doi: 10.1128/spectrum.02754-24

Figure Lengend Snippet: Carvacrol impacts C. albicans ARO1 hyphal formation. Representative epifluorescence microscopy (λ ex = 365 nm; λ em = 435 nm) images show the impact of 4 h carvacrol exposure at 1/2 MIC or MIC on C. albicans ARO1 mutant a) hyphal formation and c) pre-formed hyphae when grown in SC medium with 10% FBS ±DOX. Scale bar for the control is 5 µm and applies to all images. Bar graphs show carvacrol c) inhibits the yeast to hyphal transition and most interestingly d) reverses the pre-formed hyphal morphology for the ARO1 mutant. Statistical significance, determined by a one-way ANOVA, is denoted by ** ( P < 0.01), **** ( P < 0.0001).

Article Snippet: Morphological changes (budding yeast, germ tube, and hyphae) were identified using the transmitted light configuration of an epifluorescence microscope (λ ex = 365 nm; λ em = 435 nm; Carl Zeiss Axio Observer Z1 inverted microscope, Oberkochen, Germany) at 63×.

Techniques: Epifluorescence Microscopy, Mutagenesis, Control